Journal: Materials

Article Title: One-Minute Synthesis of Size-Controlled Fucoidan-Gold Nanosystems: Antitumoral Activity and Dark Field Imaging

doi: 10.3390/ma13051076

Figure Lengend Snippet: Optical images in dark field transmission mode under white light, of MG-63 cells, incubated ( A ) without and ( B ) with fucoidan-AuNPs. Optical images, in brightfield field reflectance mode under UV irradiation, of MG-63 cells incubated ( C ) without and ( D ) with fucoidan-AuNPs. ( E ) Show the overlay of (B) and (D). Spectra measured in several single pixels of the bright spots shown in the hyperspectral image shown in the ( F ). ( G ) and ( H ) show the hyperspectral images measured for the same sample and illumination conditions of (A) and (B), respectively. The color scale is based on the intensity of the spectra of each pixel at 750 nm. In (H), the hyperspectral image is superimposed with (D).

Article Snippet: The hyperspectral images were recorded with a hyperspectral imaging system from CytoViva, accoupled to the Olympus BX51 microscope, that includes a digital camera (Pixelfly USB, PCO) coupled to a spectrograph (V10E 2/3′’, Specim, 30 μm slit, nominal spectral range of 400–1000 nm and nominal spectral resolution of 2.73 nm).

Techniques: Transmission Assay, Incubation, Irradiation